Structural basis for chaperone-guided assembly of RNA-induced silencing complex

Data availability

The cryo-EM structure of AMC–let-7a-1 has been deposited in the PDB under accession code 9W5I . The composite map and consensus map of AMC–let-7a-1 have been deposited in the EMDB under accession codes EMD-65663 and EMD-65662 , respectively. The partial maps of local refinement focused on MID/PIWI, N-domain and HSP90–p23 have been deposited in the EMDB under accession codes EMD-65661 , EMD-65660 and EMD-65659 , respectively. The consensus map and AGO2-focused map of AMC have been deposited in the EMDB under accession codes EMD-69544 and EMD-65658 , respectively. There are no restrictions on data availability. For material requests, please contact the corresponding authors. Other structural models cited in this study for analysis ( 4W5N , 4W5O , 7KRJ , 7XW2 , 8EOB , 9CMP , 7V6C , 7ZUB , 8FFW , 5FWK , 5FWL , 7Z37 and 8GFT ) are also available at the PDB.

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